Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance.

ABSTRACT: The centromeric histone 3 variant (CENH3, aka CENP-A) is essential for the segregation of sister chromatids during mitosis and meiosis. To better define CENH3 functional constraints, we complemented a null allele in Arabidopsis with a variety of mutant alleles, each inducing a single amino acid change in conserved residues of the histone fold domain. Many of these transgenic missense lines displayed wild-type growth and fertility on self-pollination, but exhibited frequent post-zygotic death and uniparental inheritance when crossed with wild-type plants. The failure of centromeres marked by these missense mutation in the histone fold domain of CENH3 reproduces the genome elimination syndromes described with chimeric CENH3 and CENH3 from diverged species. Additionally, evidence that a singl

SUBMITTER: Kuppu S 

PROVIDER: S-EPMC4564284 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature