A predictive toxicogenomics signature to classify genotoxic versus non-genotoxic chemicals in human TK6 cells.

ABSTRACT: Genotoxicity testing is a critical component of chemical assessment. The use of integrated approaches in genetic toxicology, including the incorporation of gene expression data to determine the DNA damage response pathways involved in response, is becoming more common. In companion papers previously published in Environmental and Molecular Mutagenesis, Li et al. (2015) [6] developed a dose optimization protocol that was based on evaluating expression changes in several well-characterized stress-response genes using quantitative real-time PCR in human lymphoblastoid TK6 cells in culture. This optimization approach was applied to the analysis of TK6 cells exposed to one of 14 genotoxic or 14 non-genotoxic agents, with sampling 4 h post-exposure. Microarray-based transcriptomic analyses were

SUBMITTER: Williams A 

PROVIDER: S-EPMC4564388 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature