DNA damage in B and T lymphocytes of farmers during one pesticide spraying season.

ABSTRACT:

Purpose

The effect of one pesticide spraying season on DNA damage was measured on B and T lymphocytes among open-field farmers and controls.

Methods

At least two peripheral blood samples were collected from each individual: one in a period without any pesticide application, several weeks after the last use (January, at period P0), and another in the intensive pesticide spraying period (May or June, at period P4). DNA damage was studied by alkaline comet assay on isolated B or T lymphocytes.

Results

Longitudinal comparison of DNA damage observed at both P0 and P4 periods revealed a statistically significant genotoxic effect of the pesticide spraying season in both B (P = 0.02) and T lymphocytes (P = 0.02) in exposed farmers. In contrast, non-farmers did not show any si

SUBMITTER: Lebailly P 

PROVIDER: S-EPMC4564440 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature