Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells.

ABSTRACT:

Background and objectives

Estrogen receptor-α (ER-α) plays important roles in hepatocarcinogenesis. Recent studies have shown that ER-α could lead to cell cycle progression or inhibition of apoptosis. To better understand the role of ER-α, RNA interference (RNAi) was used to inhibit ER-α expression in the human hepatocellular carcinoma (HCC) cells.

Methods

Lentivirus-mediated ER-α small interfering RNA (siRNA) was transfected into HCC cells Hep3B. ER-α expression was monitored by real-time polymerase chain reaction (PCR) and western blot. Cell proliferation, apoptosis, and invasion were examined by methyl thiazol tetrazolium (MTT), flow cytometry (FCM), and invasion assay, respectively.

Results

ER-α siRNA efficiently downregulated the expression of ER-α in Hep3B cells

SUBMITTER: Jiang P 

PROVIDER: S-EPMC4564599 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature