Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery.

ABSTRACT:

Aim

Connexin 43 (Cx43) is a member of connexin family mainly expressed in astrocytes, which forms gap junctions and hemichannels and maintains the normal shape and function of astrocytes. In this study we investigated the role of Cx43 in astrocytes in facilitating neuronal recovery during ischemic stroke.

Methods

Primary culture of astrocytes or a mixed culture of astrocytes and cortical neurons was subjected to oxygen glucose deprivation and reperfusion (OGD/R). The expression of Cx43 and Ephrin-A4 in astrocytes was detected using immunocytochemical staining and Western blot assays. Intercellular Ca(2+) concentration was determined with Fluo-4 AM fluorescent staining. Middle cerebral artery occlusion (MCAO) model rats were used for in vivo studies.

Results

OGD/R trea

SUBMITTER: Wu LY 

PROVIDER: S-EPMC4564881 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature