Metagenomic and metatranscriptomic inventories of the lower Amazon River, May 2011.

ABSTRACT:

Background

The Amazon River runs nearly 6500 km across the South American continent before emptying into the western tropical North Atlantic Ocean. In terms of both volume and watershed area, it is the world's largest riverine system, affecting elemental cycling on a global scale.

Results

A quantitative inventory of genes and transcripts benchmarked with internal standards was obtained at five stations in the lower Amazon River during May 2011. At each station, metagenomes and metatranscriptomes were obtained in duplicate for two microbial size fractions (free-living, 0.2 to 2.0 μm; particle-associated, 2.0 to 297 μm) using 150 × 150 paired-end Illumina sequencing. Forty eight sample datasets were obtained, averaging 15 × 10(6) potential protein-encoding reads each (730 × 10

SUBMITTER: Satinsky BM 

PROVIDER: S-EPMC4564970 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature