Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling Mendelian disease.

ABSTRACT:

Rationale

An efficient and reproducible source of genotype-specific human macrophages is essential for study of human macrophage biology and related diseases.

Objective

To perform integrated functional and transcriptome analyses of human induced pluripotent stem cell-derived macrophages (IPSDMs) and their isogenic human peripheral blood mononuclear cell-derived macrophage (HMDM) counterparts and assess the application of IPSDM in modeling macrophage polarization and Mendelian disease.

Methods and results

We developed an efficient protocol for differentiation of IPSDM, which expressed macrophage-specific markers and took up modified lipoproteins in a similar manner to HMDM. Like HMDM, IPSDM revealed reduction in phagocytosis, increase in cholesterol efflux capacity and

SUBMITTER: Zhang H 

PROVIDER: S-EPMC4565503 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature