Non-Classical monocytes display inflammatory features: Validation in Sepsis and Systemic Lupus Erythematous.

ABSTRACT: Given the importance of monocytes in pathogenesis of infectious and other inflammatory disorders, delineating functional and phenotypic characterization of monocyte subsets has emerged as a critical requirement. Although human monocytes have been subdivided into three different populations based on surface expression of CD14 and CD16, published reports suffer from contradictions with respect to subset phenotypes and function. This has been attributed to discrepancies in reliable gating strategies for flow cytometric characterization and purification protocols contributing to significant changes in receptor expression. By using a combination of multicolour flow cytometry and a high-dimensional automated clustering algorithm to confirm robustness of gating strategy and analysis of ex-vivo ac

SUBMITTER: Mukherjee R 

PROVIDER: S-EPMC4566081 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature