Content of intrinsic disorder influences the outcome of cell-free protein synthesis.

ABSTRACT: Cell-free protein synthesis is used to produce proteins with various structural traits. Recent bioinformatics analyses indicate that more than half of eukaryotic proteins possess long intrinsically disordered regions. However, no systematic study concerning the connection between intrinsic disorder and expression success of cell-free protein synthesis has been presented until now. To address this issue, we examined correlations of the experimentally observed cell-free protein expression yields with the contents of intrinsic disorder bioinformatically predicted in the expressed sequences. This analysis revealed strong relationships between intrinsic disorder and protein amenability to heterologous cell-free expression. On the one hand, elevated disorder content was associated with the incre

SUBMITTER: Tokmakov AA 

PROVIDER: S-EPMC4566126 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature