Computer-Assisted Transgenesis of Caenorhabditis elegans for Deep Phenotyping.

ABSTRACT: A major goal in the study of human diseases is to assign functions to genes or genetic variants. The model organism Caenorhabditis elegans provides a powerful tool because homologs of many human genes are identifiable, and large collections of genetic vectors and mutant strains are available. However, the delivery of such vector libraries into mutant strains remains a long-standing experimental bottleneck for phenotypic analysis. Here, we present a computer-assisted microinjection platform to streamline the production of transgenic C. elegans with multiple vectors for deep phenotyping. Briefly, animals are immobilized in a temperature-sensitive hydrogel using a standard multiwell platform. Microinjections are then performed under control of an automated microscope using precision robotics

SUBMITTER: Gilleland CL 

PROVIDER: S-EPMC4566274 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature