Early insights into the potential of the Oxford Nanopore MinION for the detection of antimicrobial resistance genes.

ABSTRACT:

Objectives

Genome sequencing will be increasingly used in the clinical setting to tailor antimicrobial prescribing and inform infection control outbreaks. A recent technological innovation that could reduce the delay between pathogen sampling and data generation is single molecule sequencing. An example of this technology, which is undergoing evaluation through an early access programme, is the Oxford Nanopore MinION.

Methods

We undertook a feasibility study on six clinically significant pathogens, comparing the MinION to the Illumina MiSeq and PacBio RSII platforms. Genomic DNA was prepared and sequenced using the MinION as instructed by the manufacturer, and Illumina MiSeq and PacBio sequencing was performed using established methods.

Results

An evaluation of the ac

SUBMITTER: Judge K 

PROVIDER: S-EPMC4566964 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature