Jug r 2-reactive CD4(+) T cells have a dominant immune role in walnut allergy.

ABSTRACT:

Background

Allergic reactions to walnut can be life-threatening. Although IgE epitopes of walnut have been studied, CD4(+) T cell-specific epitopes for walnut remain uncharacterized. In particular, the relationship of both phenotype and frequency of walnut-specific T cells to the disease have not been examined.

Objectives

We sought to provide a thorough phenotypic analysis for walnut-reactive T cells in allergic and nonallergic subjects, particularly the relationship of phenotypes and frequencies of walnut-specific T cells with the disease.

Methods

The CD154 upregulation assay was used to examine CD4(+) T-cell reactivity toward the walnut allergens Jug r 1, Jug r 2, and Jug r 3. A tetramer-guided epitope mapping approach was used to identify HLA-restricted CD4(+) T-ce

SUBMITTER: Archila LD 

PROVIDER: S-EPMC4568181 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature