Ex vivo non-invasive assessment of cell viability and proliferation in bio-engineered whole organ constructs.

ABSTRACT: Decellularized organ scaffolds allow whole organ regeneration and study of cell behavior in three-dimensional culture conditions. Cell viability within the bio-engineered organ constructs is an essential parameter reflecting the performance of participating cells during long-term ex vivo culture, and is a prerequisite for further functional performance. Resazurin-based redox metabolic assays have been used to monitor cell viability in both two- and three-dimensional cell cultures. Here we developed a method for monitoring cell viability and proliferation in bio-engineered organ constructs using a resazurin perfusion assay. This method allows non-invasive, repetitive and rapid estimation of viable cell numbers during long-term ex vivo culture. As a proof-of-principle, we assessed the perfor

SUBMITTER: Ren X 

PROVIDER: S-EPMC4568186 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature