Mapping vaccinia virus DNA replication origins at nucleotide level by deep sequencing.

ABSTRACT: Poxviruses reproduce in the host cytoplasm and encode most or all of the enzymes and factors needed for expression and synthesis of their double-stranded DNA genomes. Nevertheless, the mode of poxvirus DNA replication and the nature and location of the replication origins remain unknown. A current but unsubstantiated model posits only leading strand synthesis starting at a nick near one covalently closed end of the genome and continuing around the other end to generate a concatemer that is subsequently resolved into unit genomes. The existence of specific origins has been questioned because any plasmid can replicate in cells infected by vaccinia virus (VACV), the prototype poxvirus. We applied directional deep sequencing of short single-stranded DNA fragments enriched for RNA-primed nascen

SUBMITTER: Senkevich TG 

PROVIDER: S-EPMC4568238 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature