Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.

ABSTRACT:

Background

Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped.

Methodology/principal findings

Using a combination of transcriptome profiling under various conditions and genome-wide analysis of CSL-DNA interactions, we identify genes regulated directly and indirectly by CSL proteins in fission yeast. We show that the expression of stress-response genes and genes that are expressed periodically during the cell cycle is deregulated upon genetic manipulation of cbf11 and/or cbf12. Accordingly, the coordination of mitosis and cytokinesis is perturbed in cells with genetically manipulated CSL protein levels,

SUBMITTER: Prevorovsky M 

PROVIDER: S-EPMC4569565 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature