Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.

ABSTRACT: Baker's yeast Saccharomyces cerevisiae is an attractive cell factory for production of chemicals and biofuels. Many different products have been produced in this cell factory by reconstruction of heterologous biosynthetic pathways; however, endogenous metabolism by itself involves many metabolites of industrial interest, and de-regulation of endogenous pathways to ensure efficient carbon channelling to such metabolites is therefore of high interest. Furthermore, many of these may serve as precursors for the biosynthesis of complex natural products, and hence strains overproducing certain pathway intermediates can serve as platform cell factories for production of such products. Here we implement a modular pathway rewiring (MPR) strategy and demonstrate its use for pathway optimization resu

SUBMITTER: Qin J 

PROVIDER: S-EPMC4569842 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature