Identification of characteristic TRB V usage in HBV-associated HCC by using differential expression profiling analysis.

ABSTRACT: Liver cancer is one of the most common cancers worldwide. CDR3 sequencing-based immune repertoire can be closely associated with cancer prognosis and development. Identifying the specific interaction between the TCR and cellular antigens is important for developing novel immunotherapeutic approaches for the treatment of cancer. The rearranged TCRβ loci amplified using Vβ- and Jβ-specific primers by multi-PCR and sequenced using high-throughput sequencing (HTS) in liver cancers were compared with those of T cells from healthy adult peripheral blood and from adjacent liver tissue. The T-cell repertoires within each tumor show strong similarity to one another but are distinct from those of the circulating T-cell repertoire. In addition, our results demonstrate that there are significant diffe

SUBMITTER: Han Y 

PROVIDER: S-EPMC4570125 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature