Dereplicating and Spatial Mapping of Secondary Metabolites from Fungal Cultures in Situ.

ABSTRACT: Ambient ionization mass spectrometry techniques have recently become prevalent in natural product research due to their ability to examine secondary metabolites in situ. These techniques retain invaluable spatial and temporal details that are lost through traditional extraction processes. However, most ambient ionization techniques do not collect mutually supportive data, such as chromatographic retention times and/or UV/vis spectra, and this can limit the ability to identify certain metabolites, such as differentiating isomers. To overcome this, the droplet-liquid microjunction-surface sampling probe (droplet-LMJ-SSP) was coupled with UPLC-PDA-HRMS-MS/MS, thus providing separation, retention times, MS data, and UV/vis data used in traditional dereplication protocols. By capturing these mu

SUBMITTER: Sica VP 

PROVIDER: S-EPMC4570219 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature