A Multichannel Biosensor for Rapid Determination of Cell Surface Glycomic Signatures.

ABSTRACT: Cell surface glycosylation serves a fundamental role in dictating cell and tissue behavior. Cell surface glycomes differ significantly, presenting viable biomarkers for identifying cell types and their states. Glycoprofiling is a challenging task, however, due to the complexity of the constituent glycans. We report here a rapid and effective sensor for surface-based cell differentiation that uses a three-channel sensor produced by noncovalent conjugation of a functionalized gold nanoparticle (AuNP) and fluorescent proteins. Wild-type and glycomutant mammalian cells were effectively stratified using fluorescence signatures obtained from a single sensor element. Blinded unknowns generated from the tested cell types were identified with high accuracy (44 out of 48 samples), validating the rob

SUBMITTER: Rana S 

PROVIDER: S-EPMC4571165 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature