A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis.

ABSTRACT: The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key networ

SUBMITTER: Jose M 

PROVIDER: S-EPMC4571305 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature