Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines.

ABSTRACT: The cell nucleus is a highly organized structure and plays an important role in gene regulation. Understanding the mechanisms that sustain this organization is therefore essential for understanding genome function. Centromeric regions (CRs) of chromosomes have been known for years to adopt specific nuclear positioning patterns, but the significance of this observation is not yet completely understood. Here, using a combination of fluorescence in situ hybridization and immunochemistry on fixed human cells and high-throughput imaging, we directly and quantitatively investigated the nuclear positioning of specific human CRs. We observe differential attraction of individual CRs toward both the nuclear border and the nucleoli, the former being enhanced in nonproliferating cells and the latter b

SUBMITTER: Ollion J 

PROVIDER: S-EPMC4571307 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature