Standardization of the antibody-dependent respiratory burst assay with human neutrophils and Plasmodium falciparum malaria.

ABSTRACT: The assessment of naturally-acquired and vaccine-induced immunity to blood-stage Plasmodium falciparum malaria is of long-standing interest. However, the field has suffered from a paucity of in vitro assays that reproducibly measure the anti-parasitic activity induced by antibodies in conjunction with immune cells. Here we optimize the antibody-dependent respiratory burst (ADRB) assay, which assesses the ability of antibodies to activate the release of reactive oxygen species from human neutrophils in response to P. falciparum blood-stage parasites. We focus particularly on assay parameters affecting serum preparation and concentration, and importantly assess reproducibility. Our standardized protocol involves testing each serum sample in singlicate with three independent neutrophil donors

SUBMITTER: Llewellyn D 

PROVIDER: S-EPMC4571651 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature