Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell-derived medium spiny neurons.

ABSTRACT: A systematic characterization of the spatio-temporal gene expression during human neurodevelopment is essential to understand brain function in both physiological and pathological conditions. In recent years, stem cell technology has provided an in vitro tool to recapitulate human development, permitting also the generation of human models for many diseases. The correct differentiation of human pluripotent stem cell (hPSC) into specific cell types should be evaluated by comparison with specific cells/tissue profiles from the equivalent adult in vivo organ. Here, we define by a quantitative high-throughput gene expression analysis the subset of specific genes of the whole ganglionic eminence (WGE) and adult human striatum. Our results demonstrate that not only the number of specific genes i

SUBMITTER: Straccia M 

PROVIDER: S-EPMC4571731 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature