Comparative study of proteome between primary cancer and hepatic metastatic tumor in colorectal cancer.

ABSTRACT:

Aim

To identify the differential proteins associated with colorectal cancer genesis and hepatic metastasis.

Methods

Hydrophobic protein samples were extracted from normal colorectal mucosa, primary cancer lesion and hepatic metastatic foci of colorectal cancer. With two-dimensional electrophoresis and image analysis, differentially expressed protein spots were detected, and the proteins were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry and peptide mass fingerprint analysis.

Results

Significant alterations of the proteins in number and expression levels were discovered in primary cancer and hepatic metastatic foci, the expression of a number of proteins was lost in 25-40 ku, but protein spots was increased in 14-21 ku, comp

SUBMITTER: Yu B 

PROVIDER: S-EPMC4572187 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature