A new subtype of 3' region of cagA gene in Helicobacter pylori strains isolated from Zhejiang Province in China.

ABSTRACT:

Aim

To isolate the subtypes of 3' region of cagA gene in Helicobacter pylori (H pylori) strains from Zhejiang Province in China and to investigate their relations to H pylori-associated gastroduodenal diseases.

Methods

One hundred and thirty-seven H pylori clinical strains were isolated from the gastric mucosa specimens of 74 patients with chronic gastritis, 61 with peptic ulceration, and 2 with gastric cancer. Bacterial genomic DNA was extracted and 3' region of cagA gene was amplified by polymerase chain reaction (PCR). Subtypes of 3' region of cagA gene were determined by the size of PCR amplified segments. The sequences of the subtypes were analyzed by PCR-based sequencing.

Results

Of the 137 H pylori isolates from Zhejiang Province, 132 (96.4%) yielded PCR produc

SUBMITTER: Tao R 

PROVIDER: S-EPMC4572296 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature