Three new alternative splicing variants of human cytochrome P450 2D6 mRNA in human extratumoral liver tissue.

ABSTRACT:

Aim

To identify the new alternative splicing variants of human CYP2D6 in human extratumoral liver tissue with RT-PCR and sequencing.

Methods

Full length of human CYP2D6 cDNAs was amplificated by reverse transcription-polymerase chain reaction (RT-PCR) from a human extratumoral liver tissue and cloned into pGEM-T vector. The cDNA was sequenced. Exons from 1 to 4 of human CYP2D6 cDNAs were also amplificated by RT-PCR from extratumoral liver tissues of 17 human hepatocellular carcinomas. Some RT-PCR products were sequenced. Exons 1 to 4 of CYP2D6 gene were amplified by PCR from extratumoral liver tissue DNA. Two PCR products from extratumoral liver tissues expressing skipped mRNA were partially sequenced.

Results

One of the CYP2D6 cDNAs had 470 nucleotides from 79 to 548

SUBMITTER: Zhuge J 

PROVIDER: S-EPMC4572313 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature