Growth inhibition and apoptosis induction of tanshinone II-A on human hepatocellular carcinoma cells.

ABSTRACT:

Aim

To evaluate the effects of tanshinone II-A on inducing growth inhibition and apoptosis of human hepatocellular carcinoma (HCC) cells.

Methods

The human hepatocellular carcinoma cell line SMMC-7721 was used for the study. The cells were treated with tanshinone II-A at different doses and different times. Cell growth and proliferation were measured by MTT assay, cell count and colony-forming assay. Apoptosis induction was detected by microscopy, DNA ladder electrophoresis and flow cytometry.

Results

In MTT assay, the inhibitory effect became gradually stronger with the passage of time, 24, 48, 72 and 96 h after treatment with tanshinone II-A, and the most significant effect was observed at 72 h. On the other hand, the increase of doses (0.125, 0.25, 0.5, 1.0 mg/L ta

SUBMITTER: Yuan SL 

PROVIDER: S-EPMC4572326 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature