Multiple gene differential expression patterns in human ischemic liver: safe limit of warm ischemic time.

ABSTRACT:

Aim

To investigate the multiple gene differential expression patterns in human ischemic liver and to produce the evidence about the hepatic ischemic safety time.

Methods

The responses of cells to hepatic ischemia and hypoxia at hepatic ischemia were analyzed by cDNA microarrary representing 4 000 different human genes containing 200 apoptotic correlative genes.

Results

There were lower or normal expression levels of apoptotic correlative genes during the periods of hepatic ischemia for 0-15 min, the maintenance homostatic genes were expressed significantly higher at the same time. But at the hepatic ischemia for 30 min, the expression levels of maintenance homeostatic genes were down-regulated, the expressions of many apoptotic correlative genes and nuclear transcript

SUBMITTER: Lu QP 

PROVIDER: S-EPMC4572350 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature