Increased effectiveness of carbon ions in the production of reactive oxygen species in normal human fibroblasts.

ABSTRACT: The production of reactive oxygen species (ROS), especially superoxide anions (O2 (·-)), is enhanced in many normal and tumor cell types in response to ionizing radiation. The influence of ionizing radiation on the regulation of ROS production is considered as an important factor in the long-term effects of irradiation (such as genomic instability) that might contribute to the development of secondary cancers. In view of the increasing application of carbon ions in radiation therapy, we aimed to study the potential impact of ionizing density on the intracellular production of ROS, comparing photons (X-rays) with carbon ions. For this purpose, we used normal human cells as a model for irradiated tissue surrounding a tumor. By quantifying the oxidization of Dihydroethidium (DHE), a fluoresce

SUBMITTER: Dettmering T 

PROVIDER: S-EPMC4572590 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature