The multifunctional protein CI of potyviruses plays interlinked and distinct roles in viral genome replication and intercellular movement.

ABSTRACT:

Background

The multifunctional cylindrical inclusion (CI) protein of potyviruses contains ATP binding and RNA helicase activities. As part of the viral replication complex, it assists viral genome replication, possibly by binding to RNA and unwinding the RNA duplex. It also functions in viral cell-to-cell movement, likely via the formation of conical structures at plasmodesmata (PD) and the interaction with coat protein (CP).

Methods

To further understand the role of CI in the viral infection process, we employed the alanine-scanning mutagenesis approach to mutate CI in the infectious full-length cDNA clone of Turnip mosaic virus (TuMV) tagged by green fluorescent protein. A total of 40 double-substitutions were made at the clustered charged residues. The effect of these mut

SUBMITTER: Deng P 

PROVIDER: S-EPMC4572616 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature