Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA microarray.

ABSTRACT:

Aim

To characterize the gene expression profiles associated with activation of mouse hepatic stellate cell (HSC) and provide novel insights into the pathogenesis of hepatic fibrosis.

Methods

Mice HSCs were isolated from BALB/c mice by in situ perfusion of collagenase and pronase and single-step density Nycodenz gradient. Total RNA and mRNA of quiescent HSC and culture-activated HSC were extracted, quantified and reversely transcribed into cDNA. cDNAs from activated HSC were labeled with Cy5 and cDNAs from the quiescent HSC were labeled with Cy3, which were mixed with equal quantity, then hybridized with cDNA chips containing 4000 genes. Chips were washed, scanned and analyzed. Increased expression of 4 genes and decreased expression of one gene in activated HSC were confirme

SUBMITTER: Liu XJ 

PROVIDER: S-EPMC4572762 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature