Gene expression profiles of hepatocytes treated with La(NO3)3 of rare earth in rats.

ABSTRACT: AIM:To compare the gene expression between La(NO(3))(3)-exposed and control rats in vivo. METHODS:Rats were fed La(NO(3))(3) once daily at a dose of 20 mg/kg for one month by gavage. Gene expression of hepatocytes was detected using mRNA differential display (DD) technique and cDNA microarray and compared between treated and control groups. RESULTS:Six differentially expressed sequence tags were cloned by DD, of which five were up regulated and one was down regulated in treated rats. Two sequences were determined. One band was novel. The other shared 100% sequence homology with AU080263 Sugano mouse brain mncb Mus musculus cDNA clone MNCb-5435 5'. With DNA microarray, 136 differentially expressed genes were identified including 131 over-expressed genes and 5 under-expressed genes. Most of

SUBMITTER: Zhao H 

PROVIDER: S-EPMC4572766 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature