Imbalance between the expression dosages of X-chromosome and autosomal genes in mammalian oocytes.

ABSTRACT: Oocytes have unique characteristics compared with other cell types. In mouse and human oocytes, two X chromosomes are maintained in the active state. Previous microarray studies have shown that the balance of the expression state is maintained in haploid oocytes. Here, we investigated transcripts using RNA-sequence technology in mouse and human oocytes. The median expression ratio between X chromosome and autosomal genes (X:A) in immature mouse oocytes increased as the gene expression levels increased, reaching a value of 1. However, the ratio in mature oocytes was under 1 for all expression categories. Moreover, we observed a markedly low ratio resulting from the bimodal expression patterns of X-linked genes. The low X:A expression ratio in mature oocyte was independent of DNA methylation

SUBMITTER: Fukuda A 

PROVIDER: S-EPMC4572927 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature