Molecular Methods for Identification of Acinetobacter Species by Partial Sequencing of the rpoB and 16S rRNA Genes.

ABSTRACT:

Background

Acinetobacter spp. is a diverse group of Gram-negative bacteria which are ubiquitous in soil and water, and an important cause of nosocomial infections. The purpose of this study was to identify a collection of Acinetobacter spp. clinical isolates accurately and to investigate their antibiotic susceptibility patterns.

Materials and methods

A total of 197 non-duplicate clinical isolates of Acinetobacter spp. isolates identified using conventional biochemical tests. The molecular technique of PCR-RFLP and sequence analysis of rpoB and 16S rRNA genes was applied for species identification. Antimicrobial susceptibility test was performed with a disk diffusion assay.

Results

Based on 16S rRNA and rpoB genes analysis separately, most of clinical isolates can be i

SUBMITTER: Khosravi AD 

PROVIDER: S-EPMC4572958 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature