Evaluation of an Improved Non-invasive Fetal Sex Determination in Haemophilia A Patients.

ABSTRACT:

Background

Haemophilia A (HA) is the most severe sex-linked bleeding disorder that is characterized with non-controlled and often threatening Haemorrhage. Routine fetal sex determination in early pregnancy with Haemophilia is based on invasive procedures that can be dangerous to the mother and fetus.

Aim

The goal of this study is to present an improved assay for the non-invasive fetal sex determination using a Real-Time duplex PCR on the free fetal DNA (ffDNA) obtained from the maternal serum of the HA carriers.

Materials and methods

Blood samples were eventually collected from 23 pregnant HA carriers between the 8(th) and 12(th) weeks of gestation, and after amplification by duplex-PCR of the single copy of Y chromosome-specific sequence (SRY), the product was then s

SUBMITTER: Mokari-Zadeh N 

PROVIDER: S-EPMC4572973 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature