Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides.

ABSTRACT:

Background

Flavonoids are bio-active specialized plant metabolites which mainly occur as different glycosides. Due to the increasing market demand, various biotechnological approaches have been developed which use Escherichia coli as a microbial catalyst for the stereospecific glycosylation of flavonoids. Despite these efforts, most processes still display low production rates and titers, which render them unsuitable for large-scale applications.

Results

In this contribution, we expanded a previously developed in vivo glucosylation platform in E. coli W, into an efficient system for selective galactosylation and rhamnosylation. The rational of the novel metabolic engineering strategy constitutes of the introduction of an alternative sucrose metabolism in the form of a sucros

SUBMITTER: De Bruyn F 

PROVIDER: S-EPMC4573293 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature