Patient Mutation Directed shRNA Screen Uncovers Novel Bladder Tumor Growth Suppressors.

ABSTRACT:

Unlabelled

Next-generation sequencing (NGS) of human bladder cancer has revealed many gene alterations compared with normal tissue, with most being predicted to be "loss of function." However, given the high number of alterations, evaluating the functional impact of each is impractical. Here, we develop and use a high-throughput, in vivo strategy to determine which alterations are loss of function in tumor growth suppressors. Genes reported as altered by NGS in bladder cancer patients were bioinformatically processed by MutationTaster and MutationAssessor, with 283 predicted as loss of function. An shRNA lentiviral library targeting these genes was transduced into T24 cells, a nontumorigenic human bladder cancer cell line, followed by injection into mice. Tumors that arose were seq

SUBMITTER: Hensel J 

PROVIDER: S-EPMC4573363 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature