Development and characterization of salivary adenoid cystic carcinoma cell line.

ABSTRACT:

Objective

To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.

Materials and methods

Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.

Results

Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.

Conclusion

We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

SUBMITTER: Li J 

PROVIDER: S-EPMC4573592 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature