Tissue-specific expression of histone H3 variants diversified after species separation.

ABSTRACT:

Background

The selective incorporation of appropriate histone variants into chromatin is critical for the regulation of genome function. Although many histone variants have been identified, a complete list has not been compiled.

Results

We screened mouse, rat and human genomes by in silico hybridization using canonical histone sequences. In the mouse genome, we identified 14 uncharacterized H3 genes, among which 13 are similar to H3.3 and do not have human or rat counterparts, and one is similar to human testis-specific H3 variant, H3T/H3.4, and had a rat paralog. Although some of these genes were previously annotated as pseudogenes, their tissue-specific expression was confirmed by sequencing the 3'-UTR regions of the transcripts. Certain new variants were also detected at

SUBMITTER: Maehara K 

PROVIDER: S-EPMC4574566 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature