Generation of stable mutants and targeted gene deletion strains in Cryptococcus neoformans through electroporation.

ABSTRACT: Cryptococcus neoformans is the etiologic agent of cryptococcal meningitis that causes more than half a million deaths worldwide each year. This capsulated basidiomycetous yeast also serves as a model for micropathogenic studies. The ability to make stable mutants, either via ectopic integration or homologous recombination, has been accomplished using biolistic transformation. This technical advance has greatly facilitated the research on the basic biology and pathogenic mechanisms of this pathogen in the past two decades. However, biolistic transformation is costly, and its reproducibility varies widely. Here we found that stable ectopic integration or targeted gene deletion via homologous replacement could be accomplished through electroporative transformation. The stability of the transf

SUBMITTER: Lin X 

PROVIDER: S-EPMC4574871 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature