Lung-derived exosome uptake into and epigenetic modulation of marrow progenitor/stem and differentiated cells.

ABSTRACT:

Background

Our group has previously demonstrated that murine whole bone marrow cells (WBM) that internalize lung-derived extracellular vesicles (LDEVs) in culture express pulmonary epithelial cell-specific genes for up to 12 weeks. In addition, the lungs of lethally irradiated mice transplanted with lung vesicle-modulated marrow have 5 times more WBM-derived type II pneumocytes compared to mice transplanted with unmanipulated WBM. These findings indicate that extracellular vesicle modification may be an important consideration in the development of marrow cell-based cellular therapies. Current studies were performed to determine the specific marrow cell types that LDEV stably modify.

Methods

Murine WBM-derived stem/progenitor cells (Lin-/Sca-1+) and differentiated erythroid

SUBMITTER: Aliotta JM 

PROVIDER: S-EPMC4575417 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature