Identification of novel small ncRNAs in pollen of tomato.

ABSTRACT:

Background

The unprecedented role of sncRNAs in the regulation of pollen biogenesis on both transcriptional and epigenetic levels has been experimentally proven. However, little is known about their global regulation, especially under stress conditions. We used tomato pollen in order to identify pollen stage-specific sncRNAs and their target mRNAs. We further deployed elevated temperatures to discern stress responsive sncRNAs. For this purpose high throughput sncRNA-sequencing as well as Massive Analysis of cDNA Ends (MACE) were performed for three-replicated sncRNAs libraries derived from tomato tetrad, post-meiotic, and mature pollen under control and heat stress conditions.

Results

Using the omiRas analysis pipeline we identified known and predicted novel miRNAs as well a

SUBMITTER: Bokszczanin KL 

PROVIDER: S-EPMC4575465 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature