Multi-platform assessment of transcriptional profiling technologies utilizing a precise probe mapping methodology.

ABSTRACT:

Background

The arrival of RNA-seq as a high-throughput method competitive to the established microarray technologies has necessarily driven a need for comparative evaluation. To date, cross-platform comparisons of these technologies have been relatively few in number of platforms analyzed and were typically gene name annotation oriented. Here, we present a more extensive and yet precise assessment to elucidate differences and similarities in performance of numerous aspects including dynamic range, fidelity of raw signal and fold-change with sample titration, and concordance with qRT-PCR (TaqMan). To ensure that these results were not confounded by incompatible comparisons, we introduce the concept of probe mapping directed "transcript pattern". A transcript pattern identifies probe

SUBMITTER: Yu J 

PROVIDER: S-EPMC4575490 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature