TSP-1 Deficiency Alters Ocular Microbiota: Implications for Sjogren's Syndrome Pathogenesis.

ABSTRACT:

Purpose

The potential role of commensals as triggering factors that promote inflammation in dry eye disease has not been explored. The objective of this study was to evaluate whether ocular microbiota changes with the onset of dry eye disease in thrombospondin-1-deficient (TSP-1(-/-)) mice, a strain that develops Sjögren's syndrome-like disease.

Methods

Conjunctival swabs were collected from TSP-1(-/-) and C57BL/6 mice and analyzed for bacterial presence. Opsonophagocytosis of the bacterial conjunctival isolates derived from the aged TSP-1(-/-) mice by neutrophils derived from either TSP-1(-/-) or C57BL/6 bone marrow was evaluated. The bactericidal activities of TSP-1-derived peptide were examined.

Results

We found that in TSP-1(-/-) mice, the conjunctival colonizatio

SUBMITTER: Terzulli M 

PROVIDER: S-EPMC4575514 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature