Reconstruction of conjunctival epithelium-like tissue using a temperature-responsive culture dish.

ABSTRACT:

Purpose

To study the feasibility of engineering conjunctival epithelial cell sheets on a temperature-responsive culture dish for ocular surface reconstruction.

Methods

Rabbit conjunctival epithelial cells (rCjECs) were cultured in DMEM/F-12 (1:1) medium. The morphology and phenotype of the rCjECs were confirmed with phalloidin staining, periodic acid-Schiff (PAS) staining, and immunocytochemistry. The rCjECs cultured on a temperature-responsive culture dish for 10 days produced confluent conjunctival epithelial cell sheets. Then, the phenotype, structure, and function of the conjunctival epithelial cell sheets were examined.

Results

The conjunctival epithelial cells were compact, uniform, and cobblestone shape. All cultured conjunctival epithelial cells were harvested

SUBMITTER: Yao Q 

PROVIDER: S-EPMC4575907 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature