Fast and Accurate Large-Scale Detection of β-Lactamase Genes Conferring Antibiotic Resistance.

ABSTRACT: Fast detection of β-lactamase (bla) genes allows improved surveillance studies and infection control measures, which can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited bla gene types, these methods have significant limitations, such as their failure to detect almost all clinically available bla genes. We developed a fast and accurate molecular method to overcome these limitations using 62 primer pairs, which were designed through elaborate optimization processes. To verify the ability of this large-scale bla detection method (large-scaleblaFinder), assays were performed on previously reported bacterial control isolates/strains. To confirm the applicability of the large-scaleblaFinder, the assays were perform

SUBMITTER: Lee JJ 

PROVIDER: S-EPMC4576124 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature