Toward a Whole-Cell Model of Ribosome Biogenesis: Kinetic Modeling of SSU Assembly.

ABSTRACT: Central to all life is the assembly of the ribosome: a coordinated process involving the hierarchical association of ribosomal proteins to the RNAs forming the small and large ribosomal subunits. The process is further complicated by effects arising from the intracellular heterogeneous environment and the location of ribosomal operons within the cell. We provide a simplified model of ribosome biogenesis in slow-growing Escherichia coli. Kinetic models of in vitro small-subunit reconstitution at the level of individual protein/ribosomal RNA interactions are developed for two temperature regimes. The model at low temperatures predicts the existence of a novel 5'→3'→central assembly pathway, which we investigate further using molecular dynamics. The high-temperature assembly network is incorp

SUBMITTER: Earnest TM 

PROVIDER: S-EPMC4576174 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature