Expression of Dnmt1, demethylase, MeCP2 and methylation of tumor-related genes in human gastric cancer.

ABSTRACT:

Aim

To explore the effect of DNA methyltransferase, demethylase and methyl-CpG binding protein MeCP2 on the expressions and methylation of hMSH2 and proto-oncogene in human gastric cancer.

Methods

Paired samples of primary gastric cancer and corresponding para-cancerous, non-cancerous gastric mucosae were obtained from surgically resected specimens of 28 patients. Transcription levels of Dnmt1, mbd2, MeCP2, p16(INK4A), hMSH2 and c-myc were detected by using real-time PCR or RT-PCR. Promoter methylation of p16(INK4A), c-myc and hMSH2 genes was assayed by methylation-specific PCR (MSP) and sequencing (mapping). Their relationships were analyzed by Fisher's exact test using the software SPSS.

Results

The average mRNA level of Dnmt1 gene from cancerous tissue was higher a

SUBMITTER: Fang JY 

PROVIDER: S-EPMC4576216 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature