Epstein-Barr virus in hepatocellular carcinogenesis.

ABSTRACT:

Aim

In recent years, studies have suggested that Epstein-Barr virus (EBV) is associated with HCC. The present study was to determine the prevalence of EBV in HCC patients, and whether EBV acted synergistically with hepatitis viruses in HCC carcinogenesis.

Methods

Liver tissue 115 HCC patients and 26 non-carcinoma patients were studied. Polymerase chain reaction (PCR) was performed to detect EBV BamHI W DNA, EBV LMP1 DNA, HBV X DNA, and HBV S DNA. Reverse transcription PCR (RT-PCR) was performed to detect HCV RNA and HDV RNA. Immunohistochemistry was performed to detect LMP1, HBsAg, HBcAg and HCV. The positive ratios were compared between HCC group and control group by chi2 test.

Results

Totally, 78 HCC samples whose beta-globulin DNA was positively detected by amplifi

SUBMITTER: Li W 

PROVIDER: S-EPMC4576219 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature