Methylation profile of the promoter CpG islands of 14 "drug-resistance" genes in hepatocellular carcinoma.

ABSTRACT:

Aim

To establish the DNA methylation patterns of the promoter CpG islands of 14 "drug-resistance" genes in hepatocellular carcinoma (HCC).

Methods

The methylation specific polymerase chain reaction in conjunction with sequencing verification was used to establish the methylation patterns of the 14 genes in the liver tissues of four healthy liver donors, as well as tumor and the paired non-cancerous tissues of 30 HCC patients.

Results

While 11 genes (ATP-binding cassette, sub-family G (WHITE), member 2(ABCG2), activating transcription factor (ATF2), beta-2-microglobulin (B2M), deoxycytidine kinase (DCK), occludin (OCLN), v-raf-1 murine leukemia viral oncogene homolog (RAF1), ralA binding protein 1 (RALBP1), splicing factor (45 kD) (SPF45), S-phase kinase-associated pro

SUBMITTER: Ding S 

PROVIDER: S-EPMC4576224 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature